Compounds DMSO storage as frozen solutions: dealing with difficulties

Title: Compounds DMSO Storage as Frozen Solutions: Overcoming Challenges


Dimethyl sulfoxide (DMSO) is a versatile solvent widely used in various research and industrial applications. It is commonly utilized to create frozen solutions of compounds for long-term storage, ensuring their stability and integrity. However, storing compounds as frozen DMSO solutions can present challenges, such as solubility issues and potential degradation. In this blog post, we will explore the key points surrounding the storage of compounds as frozen DMSO solutions and discuss strategies for successful storage while overcoming difficulties.

Key Points:

  1. Importance of Frozen Solutions:
    Storing compounds as frozen solutions in DMSO offers several advantages. Freezing at ultra-low temperatures (-80°C or below) can provide long-term stability, minimizing degradation and maintaining compound integrity. Frozen solutions also allow for easy and precise aliquoting, reducing the risk of repeated freeze-thaw cycles that can lead to deterioration.
  2. Solubility Challenges:
    One of the main difficulties in storing compounds as frozen DMSO solutions is achieving proper solubility. Some compounds may have limited solubility in DMSO, leading to precipitation or incomplete dissolution. It is crucial to optimize solubility by adjusting the concentration, temperature, and pH conditions. Additionally, using co-solvents (e.g., ethanol) or buffer systems can help improve solubility and stability.
  3. Preventing Compound Degradation:
    Frozen storage can protect compounds from degradation, but certain factors should be considered. Light-sensitive compounds should be stored in amber vials or wrapped in light-protective foil to minimize photochemical degradation. Temperature fluctuations should be avoided, and storage in a well-maintained ultra-low freezer is essential. Periodic monitoring of freezer performance, such as temperature and power failures, is crucial to maintaining optimal storage conditions.
  4. Proper Labeling and Documentation:
    To facilitate efficient retrieval and ensure proper usage, it is essential to label and document the frozen DMSO solutions appropriately. Each vial should be labeled with relevant information, such as compound name, concentration, date of preparation, and any specific storage requirements or precautions.
  5. Thawing and Handling:
    Thawing and handling frozen DMSO solutions require careful attention. Rapid thawing at room temperature or in a water bath can lead to temperature fluctuations, potentially affecting compound stability. It is recommended to thaw frozen solutions slowly in a controlled environment, such as in a refrigerator, and gently mix to ensure homogeneity without inducing degradation.
  6. Regular Quality Control:
    Implementing regular quality control measures is vital to assess the stability and integrity of compounds stored as frozen DMSO solutions. This can involve routine analysis of compound purity, potency, and solubility after thawing, as well as periodic visual inspection for signs of degradation.


Storing compounds as frozen solutions in DMSO is a valuable strategy for maintaining their stability and integrity over an extended period. Overcoming difficulties associated with solubility and potential degradation requires careful optimization, proper handling, and attention to storage conditions. By implementing strategies such as optimizing solubility, preventing degradation, labeling accurately, and performing regular quality control, researchers can ensure successful storage of compounds as frozen DMSO solutions. With proper execution, this method can prove advantageous in preserving valuable compounds for a wide range of research and industrial applications.